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Understanding the Signaling Pathways that Promote Oncogenesis in Cancer Cell lines
José Antonio Arellano, MD1, Yusuf Surucu, MD1, Vincent Nerone, BA1, Katherine S. Yang, BS1, Alexey V. Altman1, Rakib ul Islam1, Zayaan Trimzi1, Baris Bengur, MD; Shawn Loder, MD; Jeffrey A. Gusenoff, MD; Francesco Egro1, Asim Ejaz, PhD1
UPMC
2023-01-31
Presenter: José Antonio Arellano
Affidavit:
Peter J. Rubin
Director Name: Peter J. Rubin
Author Category: Fellow Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: Breast (Aesthetic and Recon.)
Introduction: The induction of normal cells into having a neoplastic growth has a chain of a perpetual cascade of signaling that promotes the cell to be unorganized, and unregulated and starts the proliferation of monoclonal cells, with the help of a supportive stroma that provides nutrition through blood vessels and adjacent structures. Our perfusion model was injected with oncogenic cell lines recreate this signaling to fully understand the mechanism in which a normal cell loses uniformity in size and shape and causes irreversible neoplasia, invasion of basement membrane and invades adipose tissue promoting angiogenesis.
Methods: Cancer cell lines were injected into our human perfusion system. We canulate the superficial inferior epigastric system and perfuse it with our rich media. We injected cell lines and then proceed to administer an enhancer to promote growth. After 10 days we performed an excisional biopsy to remove the tumor, afterwards we stain the samples for H&E and immunochemistry for DAPI and Ki-67.
Results: Tumor cell lines grew, and it was palpable in the physical examination of our human tissue. The H&E stain showed a rapid proliferation of cells that invaded the dermis, promoting angiogenesis in all surrounding tissues, and invading the stroma all the way to adipose tissue, there was a visible metastatic cell in distant parts of our human tissue. Immunochemistry shows the proliferation of cells.
Conclusions: Our perfusion model using human skin has shown promising results to cultivate and proliferating cancer cells to fully understand the behavior of tumor growth.
