<< Back to the abstract archive
Comparison of four BMP-based therapies for large bone defect healing in dogs
Kinsella Jr CR, Bykowski MR, Lin AY, Cray JJ, Smith DM, Lensie EL, Mooney MP, Cooper GM, Losee JE.
Division of Plastic Surgery, University of Pittsburgh Medical Center
2010-03-16
Presenter: Christopher R Kinsella Jr
Affidavit:
Director Name:
Author Category: Resident/Fellow
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Objective: The aim of this study was to compare the efficacy of rhBMP-2-mediated bone regeneration therapies with four carriers to heal large canine cranial bone defects.
Methods: Midline 3.5 x 3.5-cm cranial defects were created in twenty-three 14-week-old beagles. The animals were divided into five groups. Group A (n=3) served as a surgical control. Group B (n=5) received 0.2mg/ml rhBMP-2 in an Absorbable Collagen Sponge (ACS) carrier. Group C (n=5) received 0.4mg/ml rhBMP-2 in a Compression Resistant Matrix carrier. Group D (n=5) received 0.2mg/ml rhBMP-2 in ACS with Corticocancellous chips. Group E (n=5) received 0.2mg/ml rhBMP-2 in ACS with MasterGraft Granules. All animals are CT scanned at 0, 8, 16 and 24 weeks postoperatively. Histological evaluation occurs after animal sacrifice.
Results: Seroma ossification occurred in 18 of the 20 animals (90%) that have reached post-operative week 16 after receiving rhBMP-2 (Group B, 5/5; Group C, 5/5; Group D, 5/5; Group E, 3/5). Ectopic bone is least severe in Group E and most severe in Group C. Average defect radiopacity of subjects receiving rhBMP-2 was 99.93% by analysis of pre and post-operative 3D reconstructions (GE Viewer, Northern Eclipse). The surgical control group healed 14.6%.
Conclusions: The degree of ectopic bone formation varied across rhBMP-2 carriers. All groups treated with rhBMP-2 showed near-total defect filling on histology and CT.
