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Functional recovery of rat sciatic nerve defect repaired with tissue engineered epineural construct supported with bone marrow stromal cells: Preliminary report

Amanda Mendiola, MD; Grzegorz Brzezicki, MD; James Gatherwright, Maria Siemionow, MD, PhD
Cleveland Clinic Foundation
2010-04-01

Presenter: Amanda Mendiola

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Author Category: Resident/Fellow
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction

Objective: To engineer a BMSC epineural construct (BMSCEC) for repair of nerve defects by combining the neurotrophic effects of the epineural sheath with BMSCs. We tested regenerative potential of created in-vitro construct after implantation into the rat sciatic nerve defect.
Methods: A total of 10 SCEC were created by filling isogenic epineural sheaths with 3x106 isogenic BMSC and culturing for 10-14 days. Next in Group 1: a 2cm sciatic nerve defect was repaired with created in vitro construct. In Group 2: Standard epineural sheath filled with BMSC served as control. Animals were evaluated at 6 and 12 weeks. Nerve regeneration was assessed using SSEP, pin prick (PP), toe spread (TS) and gastrocnemius muscle index (GMI).
Results: The SCEC group showed full sensory recovery at 6 weeks. The standard repair had an average PP of 2.5 at 6 weeks and 3 at 12 weeks. The SCEC group had a better TS average of 2.3 at week 12 while the standard had 1.5. At 6 weeks, the GMI was similar between the SCEC and standard, 0.22 and 0.27 respectively. At 12 weeks, the SCEC had a better GMI of 0.61 versus 0.37. The SSEP P1 latency was 106.3% at 12 weeks for the SCEC group and 114.09% for the standard. The SCEC group showed higher amplitudes at 12 weeks, 71.5% versus 54.43% compared to standard repair.
Conclusion: We created ex vivo a new construct for repair of nerve defects which showed comparable functional recovery with our standard epineural sheath repair.

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