<< Back to the abstract archive
Harnessing the Osteogenic Capability of Neonatal Dura to Heal Critical Sized Defects
Sweeney W, Cash A, Gliniak C, Cooper G, Gosain A
The Dept. of Plastic Surgery, Case Western Reserve University, Cleveland, OH
2010-04-01
Presenter: Walter Sweeney
Affidavit:
Director Name:
Author Category: Resident/Fellow
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
In the present study we evaluated whether transplanted dura retained sufficient osteogenic potency to aid in critical size defect (CSD) repair.
Methods:Craniotomies were performed and parietal dura harvested from twenty-four Sprague Dawley rats at 8, 17, and 45 days postnatal. A 2.3 mm CSD was created in the parietal bone of twenty-four 60 day old rats. Animals were randomized into four experimental groups: A. 8-day old dura inlayed into CSD; B. 17-day old dura inlayed into CSD; C. 45 day old dura inlayed into CSD; or D. Untreated CSD. Animals were evaluated using micro-CT and radiographs. Image analysis was performed for pixel density and bone gap distance.
Results:After 5 weeks CSD's treated with 8 day old dura achieved a pixel density equivalent to undisturbed intact cranial bone (p>0.6). Untreated CSD imaged at 5 weeks achieved a pixel density 72% less than undisturbed intact cranial bone; this difference was extremely significant (p<0.0001). Post operative radiographs of animals at 8 weeks demonstrated defects treated with 8 day old dura had the smallest bone gap, 7.3 pixels. In contrast, untreated defects or defects treated with 45 day old dura had bone gaps of 37.5 and 36.4 pixels, respectively. The difference between the bone gap of animals treated with 8 day old dura was statistically significant when compared to animals treated with either 17 or 45 day old dura (p<0.05).
Conclusion:This study suggests that in vivo osteogenic properties of dura are preserved with transplantation and can be harnessed to augment CSD healing.
