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The Effects of Propranolol on VEGF Expression in a Developed In-Vitro Model of Infantile Hemangioma
Serret, M.A., Gliniak, C., Cash, A., Gosain, A.K.
Case Western Reserve University/University Hospitals of Cleveland
2010-04-03
Presenter: Marc Serret M.D.
Affidavit:
Director Name:
Author Category: Resident/Fellow
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Introduction: Regulation of endothelial cell (EC) proliferation in infantile hemangioma (IH) remains a subject of intense investigation. Previous IH culture methods listed in the literature are VEGF has been implicated as a key modulator in the process of EC proliferation. VEGF inhibition of EC proliferation in IH using corticosteroids and silencing of its expression has been highlighted for its importance in vasculogenesis i,ii . Propranolol therapy has recently been used for control of the proliferative phase of IH; the present study seeks to investigate the relationship of propranolol to VEGF expression in EC from IH.
Methods: Fresh human IH specimens obtained under a single surgeon (AG) were used to develop a reliable method of in vitro EC culture. EC isolation was performed with a CD 31 selection protocol, and was confirmed with GLUT-1 PCR expression. Proliferating IH isolates were subjected to varying exposure times (24 vs. 48 hrs) and dose levels (0.03-30mM) of propranolol. There is ongoing investigation of the effects of propranolol on VEGF expression and ß-2 receptor status in these isolates.
Results: Previous IH culture methods were found to be difficult to reproduce, prompting our lab to develop a reliable protocol that allows therapeutic mechanism investigation.
This protocol has facilitated investigation of the effects of propranolol on EC from IH.
Conclusion: A reliable EC isolation method from human IH was developed allowing further investigation of proliferation mechanisms and therapeutic inhibition.
Identification of a potential association between propranolol exposure and VEGF expression is ongoing, and further results are pending.
