<< Back to the abstract archive
Viability of the retina and optic nerve seen after whole eye transplantation in optical coherence tomography, manganese-enhanced MRI and electroretinography
Komatsu C, van der Merwe Y, He L, Miller MR, Lucy, KA, Tang H, Rosner I, Chen W, Noori J, Fu V, Steketee M, Wollstein G, Solari MG, Schuman JS, Chan KC, Washington KM. University of Pittsburgh and VA Pittsburgh Healthcare System, Pittsburgh, PA, New York University, New York, NY
University of Pittsburgh,Department of Plastic Surgery
2017-02-01
Presenter: Chiaki Komatsu
Affidavit:
I concur with the above statement.
Director Name: Kia Washington
Author Category: Fellow Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Purpose: The purpose of this study was to evaluate the viability and structural integrity of the visual system after whole eye transplantation (WET) with in vivo optical coherence tomography (OCT) , manganese-enhanced MRI (MEMRI) and electroretinography (ERG).
Methods: We performed syngeneic orthotopic WET in 9 Lewis rats. 7 rats underwent OCT at 1 week and MEMRI at 3 weeks after WET. ERG was performed at 7 weeks in 2 separate animals to evaluate retinal function.
Results: OCT revealed visualization of retinal layers in 5 of the 7 transplanted eyes. MEMRI showed significantly increased signal post-Mn injection at all the levels of visual pathway compared to pre-Mn injection. Mn-enhancement was also observed in the right, transplanted donor eye and intraorbital ON compared to pre-Mn injections. No apparent signal difference was observed between na�e and transplanted intraorbital ON post-Mn injection, or between pre- and post-Mn injection in the right recipient prechiasmatic ON, left lateral geniculate nucleus or left superior colliculus. ERG data revealed the presence small of a- and b-waves in response to light in the transplanted retinas.
Conclusions: Qualitative analysis of OCT revealed the existence of retinal layers after WET. The presence of anterograde Mn transport in the donor eye to the site of transection was seen in MEMRI. ERG data suggested a small response to light stimuli in the photoreceptors of the transplanted eyes after WET. Viability of retina and ON found in transplants allows the potential for future therapeutic strategies for neuroprotection, ON regeneration and restoration of vision.
