<< Back to the abstract archive
Effect of Calcitriol on Severely Hypoxic Adipose Tissue Survival
Sheri Wang, BS; Marisa DeSanto, BS; Alexander G Stavros, BS; Jeffrey A Gusenoff, MD; J. Peter Rubin, MD; Lauren E Kokai, PhD.
University of Pittsburgh
2019-02-15
Presenter: Sheri Wang
Affidavit:
I agree
Director Name: JP Rubin
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
BACKGROUND: While fat grafting is a powerful technique, patients must often undergo multiple procedures to achieve the desired outcome. Studies have reported up to 70% volume resorption at one year. Calcitriol, the active form of vitamin D3, is an important secosteroid in adipocyte metabolism and immune regulation. This study investigates the novel use of calcitriol for improving adipose tissue survival by reducing inflammation and phagocytic tissue clearance.
METHODS: In vitro, 200mg particles of adipose tissue from 3 donors was cultured for 48hrs in 1% oxygen. The samples were treated with 0nM, 15.6nM, 62.5nM, and 250nM calcitriol. Tissue viability was assessed. RTqPCR was used to measure adiponectin, BCL2-interacting protein-3 (BNIP3), superoxide dismutase-1 (SOD1), interferon-γ (IFNγ), and interleukin-6 (IL6) concentrations. Using a mouse model, the impact of calcitriol on fat weight and volume retention was studied. Mice in experimental groups were treated with either lipoaspirate submerged in calcitriol or intraperitoneal calcitriol injections. Control groups received no calcitriol treatment.
RESULTS: Calcitriol did not improve adipocyte viability or adiponectin expression under hypoxic conditions. However, it had decreased the expression of inflammatory cytokines SOD1, IFNγ, and IL6. In vivo, submersion of lipoaspirate in calcitriol trended towards increased fat graft retention at 1-week (p=0.0959). Intraperitoneal calcitriol injections significantly increased fat graft retention (p=0.0482) compared to control injections. The 12-week results are in progress.
CONCLUSION: Calcitriol appears to be a promising agent for reducing phagocytic clearance of grafted avascular adipose tissue.
