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Fat Grafting Does Not Increase Cancer Proliferation In Vivo
Sheri Wang, BS; Francesco Ergo, MD; Marisa DeSanto, BS; Alexander G Stavros, BS; Jeffrey A Gusenoff, MD; Lauren E Kokai, PhD; J. Peter Rubin, MD.
University of Pittsburgh
2019-02-15
Presenter: Sheri Wang
Affidavit:
I agree
Director Name: JP Rubin
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: Breast (Aesthetic and Recon.)
BACKGROUND: Fat grafting is a powerful technique in post-mastectomy reconstruction. Clinical studies have demonstrated that fat grafting does not significantly increase locoregional recurrence of breast cancer (Krastev 2019); however, results from animal studies are inconsistent. This study evaluates the effect of fat graft on tumor volume in the setting of residual breast cancer in a rodent model. We will also evaluate the potential of a localized drug delivery system using paclitaxel pellets.
METHODS: BALB/c mice were inoculated with 1x106 MCF7 (estrogen/progesterone-receptor positive) cancer cells. Beginning at 1-week, negative control mice received weekly retro-orbital paclitaxel injections. At 2-weeks, fat grafting groups received 0.3ml human lipoaspirate in mammary fat pads. In drug delivery groups, paclitaxel and estradiol pellets were implanted alongside fat. Estradiol pellets have been established as a positive control for drug release. At 8-weeks, the mice were sacrificed. Tumor volume and fat graft volume were recorded.
RESULTS: Retro-orbital paclitaxel injections, negative control, significantly decreased tumor size compared to other groups (p<0.01). Tumor volume in the fat graft groups was not significantly different than in the MCF7 only group, suggesting that fat does not increase tumor proliferation. The tumor volume in both estradiol and paclitaxel pellet groups did not significantly differ from MCF7 only. The failure of the estradiol control pellets suggests this batch of pellets were ineffective and this data cannot be used to draw conclusions regarding the paclitaxel pellets.
CONCLUSIONS: Fat grafting in the presence of residual breast cancer did not increase tumor size in the mouse model.
