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Enhancement Of Peripheral Nerve Regeneration Using Intracellular Sigma Peptide
Carlos X. Ordenana, MD1, Majid Rezaei, DDS MS1, Vahe D1, Brian Figueroa, MD1, Lynn Orfahli, BM1, Edoardo Dalla Pozza, MD1, Sayf Said, MD1, Payam Sadeghi, MD1, Francis Papay, MD1, Antonio Rampazzo, MD, PhD1, Jerry Silver, MD, PhD2, Bahar Bassiri, MD, PhD1.
1Cleveland Clinic, Cleveland, OH, USA, 2Case Western Reserve University, Cleveland, OH, USA
Cleveland Clinic Plastic surgery department
2020-02-04
Presenter: Carlos Ordenana MD
Affidavit:
I certify that the material proposed for presentation in this abstract has not been published in any scientific journal or previously presented at a major meeting. The program director is responsible for making a statement within the confines of the box below specific to how much of the work on this project represents the original work of the resident. All authors/submitters of each abstract should discuss this with their respective program director for accurate submission of information as well as the program director's approval for inclusion of his/her electronic signature.
Director Name: Bahar Bassiri
Author Category: Fellow Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
PURPOSE:
Chondroitin sulfate proteoglycans inhibit peripheral nerve regeneration through interaction with the tyrosine phosphatase ó (PTPó) receptor. Intracellular sigma peptide (ISP) is a designer peptide that blocks the PTPó receptor. In a spinal injury model, ISP administration enhanced functional recovery of locomotor and urinary systems. Our aim was to investigate the effect of ISP on peripheral nerve regeneration.
METHODS:
Thirty Lewis rats were used. Both sciatic nerves (20 mm) were procured from 10 Lewis donors.
A 10 mm defect was created in the sciatic nerve of the recipients and the isograft was interposed. The recipients received 100ľg of ISP (experimental group) or vehicle alone (control group), subcutaneously once a day. The endpoint was 90 days. Sciatic Function Index (SFI) was measured preoperatively and biweekly after surgery. Nerve conduction latency and amplitude, gastrocnemius muscle weight retention ratio and histomorphometrical nerve analysis were performed at end point.
RESULTS:
SFI declined immediately after surgery, improving gradually with the greatest value dispersion at 4 weeks. The average SFI at week 12 was -68ą19 in the ISP group and -65ą17 in the controls (p=0.64). The average conduction amplitude was 15ą8.6mV in the ISP group vs 15.9ą5.6mV in controls, while conduction latency was 2.5ą0.7ms in the ISP group vs 3.1ą2.2ms in the controls(p=0.77). The average Gastrocnemius muscle retention and axon count in the ISP group were 51.7ą10.4% and 12,381.0ą2,566.0 vs 49ą4.9%(p=0.88) and 16,204.0ą4,392.0(p=0.21) in the controls.
CONCLUSION:
Daily subcutaneous ISP did not significantly improve nerve regeneration at the dose and administration route tested.
