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Obesity and Type II Diabetes Suppresses Viability of Isolated Stromal Vascular Fraction
Phoebe L Lee, Sarah Seman, Shawn J Loder, Lauren Kokai and Peter J Rubin
University of Pittsburgh
2021-01-31
Presenter: Phoebe L Lee
Affidavit:
The project represents original work of the presenting author.
Director Name: Vu T Nguyen
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
Purpose
Adipose-derived therapeutics are abundant, minimally invasive, bioactive treatments with a range of desirable cellular and signaling functions. These effects are believed to derive primarily from the adipose stromal vascular fraction (SVF), comprised of all non-lipid laden cells. SVF is a highly clinically relevant population with the potential to be isolated and reinjected same day in patients, avoiding the need for cell culture. SVF yield and viability are often variable across patients; here we summarize our findings from SVF isolations across varying patient demographics.
Methods
Adipose tissue from 25 donors from 2019-2020 were harvested under IRB exemption. Adipose was digested with collagenase in a 1:1 ratio to yield the SVF. Cell viability and counts were determined with Trypan blue staining. Outcome measures included tissue weight, SVF yield, and viability. Patient demographics included age, sex, BMI, diabetic status, and cancer status.
Results
BMI and Diabetes predicted SVF viability. Patients with BMI>25 had significantly less SVF viability vs. those with BMI<25 (p<0.01). Diabetics patients also demonstrated significantly reduced SVF viability compared to non-diabetics (p<0.05). BMI>25 and diabetes were also found to be positively correlated (p<0.01). Sex, age, and cancer status had no significant impact on SVF viability or yield per tissue weight.
Conclusions
BMI and diabetic status impacted SVF yield and viability the greatest. These variables may be used to predict SVF harvest in patients undergoing ASC cell-based therapy.
