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Transforming Growth Factor Beta 1 Improves Bone Healing And Promotes Suture Regeneration
Shakir S, MacIsaac ZM, Naran S, Gilbert JR, Shaw MA, Yu HY, Wang D, Losee JE, Cooper GM
University of Pittsburgh, Department of Plastic Surgery, Craniofacial Biology Laboratory
2013-03-13
Presenter: Sameer Shakir
Affidavit:
I certify that the material proposed for presentation in this abstract has not been published in any scientific journal or previously presented at a major meeting. This work is that of the author's in its entirety.
Director Name: Joseph E. Losee
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
BACKGROUND: Repair of complex cranial defects is hindered by a paucity of appropriate donor tissue. BMP2 and TGFb1 have been shown separately to induce bone formation and potentially improve surgical outcome for cranial defect repair by obviating the need for donor tissue. We hypothesized that combining BMP2 and TGFb1, using inkjet-based biopatterning, would augment cranial repair within calvarial defects.
METHODS: Suturectomies (3×15mm) were performed in 10-day-old New Zealand White rabbits (n=19). Acellular dermal matrix (3x15mm) patterned with 4 treatments (vehicle, 350ng BMP2, 200ng TGFb1, or 350ng BMP2 + 200ng TGFb1) was placed in suturectomy sites and rabbits were euthanized 4 weeks post-surgery. Bone healing was quantified by computerized tomography (CT). Regenerated bone was qualitatively assessed histologically.
RESULTS: One-way ANOVA revealed significant differences between the groups (F=4.881). Bonferonni posthoc analysis revealed significant differences between the control group and treatment groups, but no significant differences were detected among treatment groups. The TGFb1 treatment group produced bone with cellularity and matrix density most similar to native bone. Pentachrome staining demonstrated cartilage in the BMP2-regenerated bone, which was not present in other treatment groups.
CONCLUSIONS: Growth factor delivery using inkjet-based biopatterning augments cranial repair. Total healing in BMP2 and TGFb1 treatment groups is not significantly different. The combination of BMP2 + TGFb1 did not significantly increase bone healing compared to treatment with BMP2 or TGFb1 alone. TGFb1 improved bone formation without producing a cartilaginous intermediate, more closely resembling the intramembranous ossification pathway native to calvarial bones.
