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Cryopreserved Calvarial Bone Healing After Cranioplasty In Mice
Abdulaziz Elemosho, MD, Jude C. Kluemper, BS, Farah A. Al-Omari, PhD, Caroline Gillespie, BS, Ryan Enslow, BS, Hallie Harris BS MCR, James E. Orfila, PhD, Paco S. Herson, PhD, Do-Gyoon Kim, PhD, Kerry-Ann S. Mitchell, MD PhD FACS.
The Ohio State University
2025-01-10
Presenter: Abdulaziz Elemosho
Affidavit:
I certify that the work presented here is the original work of Zed and other members of the Mitchell Lab. And this work has not been presented at any other meeting
Director Name: Gregory Pearson MD
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Introduction
Cranioplasty with autologous cryopreserved calvarial bone grafts (CCBGs) has a high risk of complications. The goal of this study was to utilize a mouse model to delineate the process of calvarial bone healing.
Methods Two groups of adult C56BL/6 mice underwent hemicraniectomy. One group had cranioplasty with non-cryopreserved calvarial bone graft (nCCBG), while the other group had cranioplasty with CCBG. Six cohorts per group, with at least eight mice each group, were euthanized at 1, 2, 3, 4, 8, and 10 weeks post-cranioplasty. Immunohistochemistry and Micro-CT were performed to evaluate healing.
Results
Different regions were evaluated: 1) central bone graft, 2) bone graft and 3) defect rim (native bone) to evaluate the healing process at different regions after cranioplasty. DAPI intensity and nuclei counts indicated a significantly higher cell density in the bone grafts (p=0.0031) of nCCBG group from weeks 1 to 4, compared to the CCBG group. No differences were observed in native bone densities between groups. Vascular intensity via lectin staining was similar across groups and timepoints. Micro-CT analysis showed that the bone graft rim had a significantly higher remodeling activity than the central bone graft in nCCBG (p=0.033) and CCBG (p<0.0001) groups regardless of timing, and similar remodeling activity was noted between the defect rim and bone graft rim regardless of cranioplasty type.
Conclusions
Cranioplasty with CCBG is accompanied by decellularization of bone graft, when compared to nCCBG. Further studies are required to understand the mechanism of this bone loss and resorption following cranioplasty with CCBG
