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Bone Marrow Derived Stromal Cells (BMDSC) Therapy and their neurotrophic activity in Peripheral Nerve Regeneration
Maria Madajka, Amanda Mendiola,Beta Przybyla, Maria Siemionow
Cleveland Clinic
2010-03-30
Presenter: Maria Madajka
Affidavit:
Director Name:
Author Category: Resident/Fellow
Presentation Category: Basic Science Research
In the USA there are over 50,000 peripheral nerve repair procedures yearly. Techniques such as cable nerve grafts or conduits from biodegradable polymers have limitations due to postoperative complications. In our research we investigate two objectives:1) To develop a new surgical protocol with the use of epineural sheath filled with BMSC 2) To characterize the neurotrophic capabilities of new conduit together with the multipotential capabilities of BMSC. Methods: Epineural sheaths were filled with stained BMSC and the level of released NGF into medium was measured. Constructs were than implanted into LEW rats and collected nerve samples were examed histologically. Results: NGF release was detected in vitro after 4 days in culture in case of epineurial sheats filled with BMSC and reached the maximum level of 1000pg/mL by day 10. Cultures of only BMSC or epineural sheaths had 2 weeks delay in the release of NGF, which was detected at the concentration range of 30-200pg/mL. Immunohistochemistry revealed that expression of H-neurofilaments was detectable 6 weeks after implantation. CD31 positive cells were present inside of epineural tube and co-localized with dividing cells. The presence of VEGF, GFAP and S-100 was also detected. Conclusions: Epineural sheath filled with BMSC has a high capacity to maintain secretion of NGF, which could be the most critical neutrotrophic factor. The growth of neurofilaments in the lumen of the tube indicates successful nerve growth. The presence of endothelial markers on the surface of dividing cells inside the construct reflects self-maintenance of their pro-regenerative activity.
