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Adipose derived stem cell proliferation and VEGF secretion is increased by wound fluid stimulation
Christine Fisher MD, Joseph Michaels V MD, Jedidiah McAtee, Mostafa Ramadan MD, Natasa Miljkovic MD, Han Li MD, Kacey Marra PhD, J. Peter Rubin MD
University of Pittsburgh
2010-04-05
Presenter: Christine Fisher, MD
Affidavit:
Director Name:
Author Category: Resident/Fellow
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
INTRODUCTION: Mesenchymal stem cells have tremendous therapeutic potential to promote wound healing, given their ability to differentiate into multiple cell types and to express multiple growth and angiogenic factors.
BACKGROUND: Bone marrow derived mesenchymal stem cells have been shown to enhance healing, but their clinical use is limited by a low stem cell yield per harvest and donor morbidity. Given the autologous and readily available nature of adipose derived mesenchymal stem cells, there is tremendous unexplored potential in the treatment of difficult to heal wounds.
HYPOTHESIS: Adipose derived stem cells (ASCs) grown in media primed with wound exudates from split thickness skin graft donor sites will show increased proliferation and vascular endothelial growth factor (VEGF) secretion.
RESEARCH: ASCs were cultured in media primed with wound fluid collected at 24 and 48 hours, serum, and regular growth media for 48 and 72 hours in aerobic and anaerobic conditions. CyQuant and ELISA quantified the number of cells and measured the amount of VEGF present at each time point.
OBSERVATIONS: Primed ASC secreted 4.5 times more VEGF than ASC grown in hypoxic media (p=0.00002), and 1.8 times more VEGF than ASC primed with serum (p=0.011). Cell proliferation was similar in the 48 and 72 hour groups, but was greater than growth in serum (p=0.13) or hypoxic media (p=0.11).
CONCLUSIONS: ASC primed with fresh wound exudates show increased proliferation and VEGF secretion. The in vitro data supports efficacy of primed ADSCs in enhancing wound healing and demonstrates the translational potential of this therapy.
