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Collagen type XI á1 facilitates head and neck squamous cell cancer growth and invasion
J C Sok, J A Lee, S Dasari, S Joyce, S C Contrucci, A M Egloff, B K Trevelline, R Joshi, N Kumari, J R Grandis and S M Thomas
University of Pittsburgh
2014-03-14
Presenter: J A Lee
Affidavit:
Director Name:
Author Category: Resident Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Background:
Although it is well established that the extracellular matrix affects tumour progression, not much is known about the various components and their effect on head and neck squamous cell carcinoma (HNSCC) progression. Levels of collagen type XI á1 (colXIá1), a minor fibrillar collagen, have been shown to be increased in tumour compared with normal tissue in several cancers, including colorectal, breast, and non-small cell lung cancer. Currently, the functional significance of colXIá1 is not understood.
Methods:
We examined the expression levels of colXIá1 mRNA and elucidated the functional role of colXIá1 in HNSCC. Cell proliferation, invasion, and migration were examined with and without colXIá1 knockdown with siRNA in HNSCC cells.
Results:
Our data demonstrate that colXIá1 expression is increased in tumour samples compared with levels in normal adjacent tissue in 16/23 HNSCC patients. In addition, colá11 is increased in HNSCC cell lines compared with normal immortalised epithelial cells and is increased in tumour-derived fibroblasts compared with normal fibroblasts. Using an siRNA approach, we demonstrate that colXIá1 contributes to proliferation, migration, and invasion of HNSCC.
Conclusion:
Our cumulative findings suggest that colXIá1 contributes to HNSCC tumorigenesis and may serve as a potential therapeutic target.
