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The Effectiveness of Human Amnion Derived Materials in Acute Wound Healing
Cagri Cakmakoglu, M.D, Addison Barnett, B.S, Maria Madajka, PhD, Karolina Mlynek, M.D, Stephanie Kortyka, M.D, Julia Maxey, B.S, Russell Frautschi, B.S, Brianna Halasa, B.S, James Zins, M.D
Cleveland Clinic Plastic Surgery Department
2016-02-01
Presenter: Cagri Cakmakoglu, M.D
Affidavit:
%90 of the work on this project represents the original work of the resident.
Director Name: James Zins
Author Category: Resident Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
Abstract:
Purpose:The aim of this study is to evaluate the efficacy of three differently processed, human amnion derived materials in enhancing acute wound healing in a rat model.
Methods: Two full-thickness wounds, a control and experimental, were created on the dorsum of each Lewis rat. Rats were divided into 3 groups by product: (Group1,n=16) Dehydrated membrane, (Group2,n=16) fresh hypothermically stored membrane, (Group3,n=12) Cryopreserved membrane. Wounds were analyzed at 2 and 6 weeks for percentage of wound closure, trans-epidermal water loss (TEWL), and tensile strength statistically assessed by a paired two-tailed t-test.
Results: Mean percentage of wound closure at 2 weeks in groups 1, 2, and 3, respectively, were 82.4%,81.6%,82.9% (experimental) and 81.9%,85.7%,86.9% (control) (p=0.89,0.20,0.40); and at 6 weeks were 92.0%,92.7%,94.0%(experimental) and 93.1%,90.7%,92.3% (control) (p=0.29,0.06,0.45). Mean values of TEWL [g/m2/hr] at 2 weeks were 60.2,52.1,58.8 (experimental) and 50.3,30.3,56.0 (control)(p=0.61,0.08,0.88); and at 6 weeks were 6.1,16.7,3.9 (experimental) and 5.9,5.5,7.2 (control) (p=0.92,0.15,0.01). Mean tensile strength values [Newtons] at 2 weeks were 4.4,3.9,4.6 (experimental) and 4.8,5.0,5.2 (control) (p=0.57,0.14,0.23); and at 6 weeks were 11.3,9.3,9.0 (experimental) and 10.5,9.9,9.1 (control) (p=0.41,0.44,0.74).
Conclusions: This study did not demonstrate differences, however, there were limitations in its design: a critical size defect could not be created due to mortality, measurement technique sensitivity may not reflect healing enhancement, and immunohistochemical and histological analysis is pending. Further animal model studies employing different techniques need to be performed
