<< Back to the abstract archive
Harnessing "Sterile Inflammation" to Improve Demineralized Bone Matrix Remodeling
Bykowski MR, Wang D, Taylor GM, Losee JE, Cooper GM
University of Pittsburgh Medical Center, Department of Plastic Surgery
2016-02-01
Presenter: Michael R Bykowski
Affidavit:
The resident was responsible for the majority of the work described in this abstract.
Director Name: Joseph Losee
Author Category: Resident Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: Craniomaxillofacial
Background and Purpose: Demineralized bone matrix (DBM) is used in approximately 20% of bone-grafting procedures as a graft extender or as a stand-alone graft material. However, the clinical performance of commercial DBM is extremely variable, necessitating a need for improved performance of DBM in reconstructive surgeries. Damage associated molecular patterning proteins (DAMPs) are endogenous proteins released in response to tissue injury. The ability of DAMPs to promote remodeling of DBM implants and improve graft-induced bone regeneration was studied.
Method/Description: Circular DBM implants treated with PBS (control) or S100A8 and S100A9 (DAMPs) were placed above 5mm round calvarial defects generated in mature mice (16 weeks old). Implants were analyzed at 1 or 2 months post procedure. DBM remodeling was measured by histology (H&E, TRAP, and osteopontin [OPN] staining).
Results: Viability and remodeling of DBM was estimated by counting the number of nuclei per unit area of bone tissue within the defect. We found that DBM had 3.3 times greater nuclei density in the DAMP treated implants versus the control. Evidence of increased bone resorption in the DAMP treated DBM implants was detected by intense mouse OPN staining and increased number of osteoclasts (TRAP staining) compared to the control. Preliminary computed tomographic studies demonstrate that DAMP-treated DBM improves bone regeneration at 1-month and 2-month time points.
Conclusions: These data suggest that the presence of DAMPs improved remodeling of DBM implants. Further studies must be performed to determine regenerated DBM volume.
