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The Effects of Cold Storage and Poloxamer 188 Treatment on Stromal Vascular Fraction Viability and Volume Retention of Fat Grafts
Gabriella DiBernardo, Jacqueline Bliley, Debra Bourne, Emmanuel Havis, Isaac James, Ryan Schroth, Aaron Dees, Damian Grybowsky, Sheri Wang, Lauren Kokai, Arta Kelmendi-Doko, Chris Mahoney, Wes Sivak, Kacey Marra, J. Peter Rubin
University of Pittsburgh, Department of Plastic Surgery
2016-02-01
Presenter: Gabriella DiBernardo
Affidavit:
The submitted abstract has not been previously present at a major meeting or published in any scientific journal. The submitted work represent the original work of the submitter.
Director Name: Not a resident
Author Category: Medical Student
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
Background: Fat grafting is used for restoring soft-tissue defects, including those resulting from breast resection and craniofacial trauma. Fat ischemia secondary to harvest leads to unpredictable retention. Cold storage is commonly used and is believed to reduce tissue damage during ischemia. Poloxamer-188 has been used to prevent apoptosis and increase graft survival. We aim to determine whether cooling with P188 could increase graft retention.
Methods: Fat was obtained from surgical patients by liposuction and subsequently, incubated at 4C, room temperature, 4C+P188 and RT+P188 for various time points. The Stromal Vascular Fraction was isolated at each time point to assess cell yield and viability. Lipoaspirate samples were collected to assess gene expression of apoptotic, angiogenic and senescent factors. Treated fat grafts were injected onto flanks of athymic nude mice and explanted 6 weeks postoperatively to assess volume retention and tissue architecture.
Results and Conclusions: Viability of the SVF was decreased in RT and 4C groups at 4.5 hours. P188 appeared to preserve cell viability with both treatments. Gene expression indicated 4C treatment had downregulated p21 and PPAR-γ expression at 7.5 and 24 hours compared to RT groups. A downregulation of angiogenic factors was observed at 24 hours with 4C treatment. Volume retention at 6 weeks had no significant difference observed between groups. A more inflammatory histological appearance was observed in 4C treated groups. Histology indicates increased adipocyte viability in RT and P188 treatments. These results suggest cooling negatively impacts SVF and adipocyte viability, gene expression, and histological appearance of transplanted grafts.
