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Iontophoresis Delivery System (Ion VAC) as a New Drug Delivery Platform to Reduce Biofilm in Porcine Skin Burn Model –Preliminary Report.
Maria Madajka PhD, Karolina Mlynek MD, Edoardo Dalla Pozza MD, Kihyun Cho MD, Nadeera Dawlagala MD, Hirsh Shah BA, Vahe Fahradyan MD, Grzegorz Kwiecien MD, Christine McDonald PhD, Craig R.Homer MS , Bahar Bassiri Gharb MD PhD, Frank Papay MD
Cleveland Clinic
2017-02-14
Presenter: Maria Madajka
Affidavit:
This project is the original work of the research fellow
Director Name: Bahar Bassiri Gharb
Author Category: Fellow Plastic Surgery
Presentation Category: Basic Science Research
Abstract Category: General Reconstruction
Introduction: The goal of this study was to assess the antimicrobial properties of the wound healing technology that combines iontophoretic delivery of silver ions with negative pressure (Ion VAC). Iontophoresis employs an electric current to transfer antimicrobial silver ions within wounds. The delivery platform is composed of a set of electrodes connected through a TENS unit which regulated the electrical parameters.
Materials and Methods: Eight dermal contact burns measuring 5 x 5cm were created on the back of Yorkshire pig. A burn device was preheated to 150°C and applied to the skin for 1 minute using a pressure of 6.0 N/cm2. After 7 days of natural biofilm development, the Ion-Vac system was applied on the surface of four wounds. The remaining wounds served as a non-treated control. The bacterial load (CFU) was measured on the wound surface and in homogenized biopsies before the treatment as well as days 1, 7, 11, 14, 15, 22 and 24 after the Ion-VAC application.
Results: There was significantly reduced bacterial growth up to the day 14 of the IonVAC treatment when compared to non-treated wounds. No significant decrease was detected in the CFU number at days 15, 22 and 24. Silver concentration was determined by mass spectrometry in the entire wound area ranging from 8 to10 ́g/g. No preferable silver localization site was detected in the wound area.
Conclusions: IonVAC system inhibits bacterial growth on full thickness skin burns till 14 days of therapy. Further analysis of electro-chemical properties of Ion-VAC are under investigation.
