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The Efficacy of Human Amnion Derived Biologic Materials in an Acute Wound Healing Model
Karolina Mlynek MD, MBA, Maria Madajka PhD, Cagri Cakmakoglu, MD, Kashyap Tadisina
MD, Stephanie Kortyka, MD, Addison Barnett, BS, Julia Maxey, BS, Russell Frautschi,
BS, Brianna Halasa, BS, James E. Zins, MD, FACS
Cleveland Clinic
2017-02-15
Presenter: Karolina Mlynek or other
Affidavit:
This is the original work of the authors mentioned in title
Director Name: Bahar Bassiri Gharb MD PhD
Author Category: Other Specialty Resident
Presentation Category: Clinical
Abstract Category: General Reconstruction
Background:
The objective of this study was to evaluate the efficacy of three differently processed, human amnion derived biological dressings in enhancing acute wound healing in a rat model. Amnion derived products can be easily produced and serve as an alternative to currently used dressings.
Materials and Methods:
A total of 72 inbred male Lewis rats were divided into three groups (n=24 each) based on the products used: Group 1 - dehydrated amnion/chorion membrane; Group 2 - fresh hypothermically stored amniotic membrane; Group 3- cryopreserved amniotic membrane.
Each group was further divided into 2 sub-groups based upon scarification time of 2 and 6 weeks. Two full thickness, 2x2 cm skin defects were created on the rat dorsum. One wound served as non-treated control and one as experimental side. Amniotic products application was performed immediately after the wound creation. The percentage of wound closure, transepidermal water loss, tensile strength measurements and histological analysis were performed on POD 3, 7 and then on weekly basis.
Results:
No statistically significant difference in the percentage of wound closure was found in Groups 1 and 2. No statistically significant difference was found in transepidermal water loss in all groups. The evaluation of tensile strength at 6 weeks showed no statistically significant difference in Groups 1 and 3, however, it reached higher values in the experimental wounds from Group 3.
Conclusion:
Only the cryopreserved amniotic membrane group at day 3 was statistically and clinically significant. Further animal model studies employing different techniques need to be performed.
